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Digital holographic microscopy (DHM) is digital holography applied to microscopy. Digital holographic microscopy distinguishes itself from other microscopy methods by not recording the projected image of the object. Instead, the light wave front information originating from the object is digitally recorded as a hologram, from which a computer calculates the object image by using a numerical reconstruction algorithm. The image forming lens in traditional microscopy is thus replaced by a computer algorithm. Other closely related microscopy methods to digital holographic microscopy are interferometric microscopy, optical coherence tomography and diffraction phase microscopy. Common to all methods is the use of a reference wave front to obtain amplitude (intensity) and phase information. The information is recorded on a digital image sensor or by a photo detector from which an image of the object is created (reconstructed) by a computer. In traditional microscopy, which do not use a reference wave front, only intensity information is recorded and essential information about the object is lost. Digital holography has mostly been applied to light microscopy. However, digital holography has also been applied to electron microscopy. Holography was invented by Dennis Gabor to improve the electron microscope. For various reasons holography never made it into the electron microscope. == Working principle == To create the necessary interference pattern, i.e., the hologram, the illumination needs to be a coherent (monochromatic) light source, a laser for example. As can be seen in Figure 2, the laser light is split into an object beam and a reference beam. The expanded object beam illuminates the sample to create the object wave front. After the object wave front is collected by a microscope objective, the object and reference wave fronts are joined by a beam splitter to interfere and create the hologram. Using the digitally recorded hologram, a computer acts as a ''digital lens'' and calculates a viewable image of the object wave front by using a numerical reconstruction algorithm. Commonly, a microscope objective is used to collect the object wave front. However, as the microscope objective is only used to collect light and not to form an image, it may be replaced by a simple lens. If a slightly lower optical resolution is acceptable, the microscope objective may be entirely removed. Digital holography comes in different flavors, such as ''off-axis Fresnel'', ''Fourier'', ''image plane'', ''in-line'', ''Gabor'' and ''phase-shifting'' digital holography, depending on the optical setup. The basic principle, however, is the same; a hologram is recorded and an image is reconstructed by a computer. The lateral optical resolution of digital holographic microscopy is equivalent to the resolution of traditional light microscopy. DHM is diffraction-limited by the numerical aperture, in the same way as traditional light microscopy. However, DHM offers a superb axial (depth) resolution. An axial accuracy of approximately 5 nm has been reported. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「digital holographic microscopy」の詳細全文を読む スポンサード リンク
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